Diff-Quik Stain (For Auto Stainer) Baso Clear Colourful Stains

Testing Principle

Proteins within cells and sperm, which have different isoelectric points, carry varying charges under the same acidity conditions, enabling them to selectively bind to corresponding dyes and become stained. Acidophilic proteins dissociate to form amino groups with positive charges, which can bind to the negatively charged acidic dye (eosin) and appear red. Basophilic proteins dissociate to form carboxyl groups with negative charges, which can bind to the positively charged basic dye (methylene blue) and appear blue. Neutrophilic proteins dissociate to form equal amounts of positively charged amino groups and negatively charged carboxyl groups, simultaneously binding equal amounts of acidic and basic dyes, resulting in a purple-red color. However, due to the equal dissociation of charges, the staining is relatively weak. This staining method is the WHO-recommended DiOuik staining technique.

Stain Results

1.Blood Smear Staining

Red blood cells appear red,Granules in the cytoplasm of white blood cells are clearly visible, displaying the unique colors characteristic of each cell type. Cell nuclei are stained purple-red, with clearly visible chromatin structures.

2.Semen Smear Staining

The post-acrosomal region of the sperm head appears purple-blue, while the acrosomal region appears light purple-red.The midpiece and tail of the sperm are stained light red.Various cells in the semen exhibit different morphologies and staining results due to their distinct shapes and compositions.

Packaging Specification

Wuhan Jinhong Biotech Development Co., Ltd is the sales center of Baso products and also as an applicant and manufacturer of CE certificate of Plasma Freezing System with Intelligent Temperature Control. Wuhan Jinhong, since 1993, is a very professional team with a sales network covering 31 provinces, municipalities and autonomous regions across the country. At the same time, it has long-term cooperation with agents, distributors and companies to ensure customers enjoy high efficiency, high quality post-sales service.

Baso Group commit to continuous innovation and quality improvement, and constant effort to reach the level of the world's first. We hope to share the good quality to every customer around the world.